Toll样受体4及核因子-κBp65在口腔扁平苔藓中的表达及意义

Expression of Toll like receptor 4 and nuclear factor-kappa Bp65 in oral lichen planus

目的:探讨Toll样受体4(toll like receptor 4,TLR4)、核因子-κBp65(nuclear factor-kappaB,NF-κBp65)在口腔扁平苔藓(oral lichen planus,OLP)病损形成及发展中的作用。方法:采用SP免疫组化方法检测35例OLP及10例正常口腔黏膜中TLR4、NF-κBp65的表达情况,分析两者的相关性及其与OLP临床病理特征的关系。结果:OLP病损组织中TLR4、NF-κBp65的阳性率分别为66%(23/35)、89%(31/35),在正常黏膜组分别为30%(3/10)和40%(4/10),组间比较P<0.05。TLR4和NF-κBp65在OLP病损组织中的表达为正相关关系(P<0.05)。NF-κBp65在网纹型OLP中的表达水平明显高于在萎缩-糜烂型OLP中的表达(P<0.05)。结论:TLR4和NF-κBp65在OLP病损形成中有重要意义。

Objective： To investigate the possible role of Toll like receptor 4 （TLR4） and nuclear factor- kappaBp65 （ NF- κBp65 ） in oral lichen planus（OLP）. Methods： Immunohistochemical technique SP method was used to detect the expression of TLR4 and NF- κBp65 in 35 OLP cases and 10 normal oral mucosa specimens. The correlation and their respective relationship with clinical features of OLP were statistically analyzed. Results： The positive rates of TLR4 and NF-κBp65 were 66% （23/35） and 89% （31/35） in OLP cases, 30% （3/10） and 40% （4/10） in normal controls, respectively（case vs control, P 〈0.05）. There was a positive corre- lation between the expression of TLR4 and NF-κBp65 in OLP tissues （P 〈 0.05 ）. Expression of NF-κBp65 was significantly higher in the patients with atrophic- erosive form （P 〈 0.05 ）. Conclusion： The over expression of TLR4 and NF- κBp65 in OLP may play a role in the pathogenesis of OLP.