1.0%与0.2%葡萄糖环境中口腔变形链球菌基因表达的差异

Detection of Differentially Expressed Genes of Streptococcus Mutans Cultured in 1.0% and 0.2% Glucose

目的观察1.0%与0.2%葡萄糖营养条件对变形链球菌基因表达的影响。方法变形链球菌分别在1.0%和0.2%葡萄糖条件下培养,利用前期构建的变形链球菌基因表达谱检测芯片筛选两种培养条件下变形链球菌中差异表达的基因,以观察环境葡萄糖供给情况对变形链球菌基因表达的影响。结果相对于0.2%葡萄糖,在1.0%葡萄糖条件下变形链球菌中有57种基因表达发生显著变化,其中与变形链球菌糖转运系统和能量代谢、黏附等细菌毒力、DNA复制、重组、修复、信号传递与调控系统、细菌表面成分等相关的41种基因表达明显上调,而一些参与细胞生理过程的感受态蛋白和酶的16种基因表达显著下调。结论不同葡萄糖供给情况影响变形链球菌多种基因的表达。

ObJective To detect the differently expressed genes Of Streptococcus mutans （S. mutas ） cultured in 1.0% glucose and 0.2% glucose. Methods S. mutans was cultured in 1.0% and 0.2% glucose. The genetic expression of S. mutans under different conditions was detected using gene chips buih previously. Results In 1.0% glucose environment, expressions of 57 S. mutans genes significantly changed compared with 0.2% glucose. Forty-one genes associated with sugar transport and energy metabolism, virulence, pathogenesis, DNA metabolism and regulatory functions were significantly up-regulated. While 16 genes encoding some competence proteins and enzymes related to cellular processes were significant- ly down-regulated. Conclusion The environmental glucose affects multiple gene expressions of S. mutans.