铅中毒者T细胞受体-CD3分子复合物基因表达模式

Change of T cell TCR-CD3 complex-mediated gene expression pattern in lead poisoning patients

目的了解铅中毒T细胞TCR—CD3分子复合物中各组成基因（CD37、CD38和CD3ε）的表达特点。方法采用SYBRGreenI荧光定量PCR法检测46名铅中毒者（11名观察对象组、35名轻度铅中毒组）及31名对照组外周血单个核细胞中CD3各亚单位表达情况。结果铅中毒观察对象组及轻度铅中毒组中CD3γ基因表达中位数水平分别为6．89％、5．87％，与对照组（3．78％）相比，明显升高，差异有统计学意义（P〈0．05）；铅中毒观察对象组、轻度铅中毒组中CD38基因表达中位数水平分别为0．54％、0．70％，较照组相比（1．03％）明显下降，差异有统计学意义（P〈0．05）；铅中毒观察对象组及轻度铅中毒组中CD3ε基因表达中位数水平分别为10．22％、6．08％，与对照组（3．52％）相比明显升高，差异有统计学意义（P〈0．05）。铅中毒者CD3γ、CD3ε与工龄呈正相关，差异有统计学意义（r==0．371，P〈0．05；r=-0．367，P〈0．05）。铅中毒者CD3γ、CD3ε与血ZPP、尿8-ALA浓度呈负相关，差异有统计学意义（r=-0．358，P〈0．05；r=-0．385，P〈0．05）。采用偏相关分析方法，控制血铅、尿铅因素后CD3ε基因表达水平与血ZPP、尿δ-ALA则无相关关系（r=0．101，P〉0．05）。CD3各基因在对照组中的表达水平从高至低顺序依次为CD3γ、CD3ε、CD38，而在铅中毒观察对象组和轻度铅中毒组均为CD3ε、CD3γ、CD38。结论铅中毒者中TCR—CD3分子复合物组成基因表达水平发生改变，其变化可能与机体的T细胞免疫功能受损有关，CD3e基因表达水平可作为铅中毒免疫功能筛查敏感指标。

Objective In order to study the feature of T cell TCR-CD3 complex-mediated gene in lead poisoning patients. Methods Real-time PCR with SYBR Green I technique was used for determination of the expression levels of CD3 genes in peripheral blood mononuclear cells of 46 cases lead poisoning patients （11 cases in observation group and 35 cases in mild lead poisoning group） and 31 cases in control group. Results The median expression levels of CD3γgene in observation group and mild lead poisoning group （6.89%,5.87 %）were higher than the control group （P〈0.05）. The median expression levels of CD3δ gene ila observation group and mild lead poisoning group （0.54%, 0.70%） were lower than the control group （P〈0.05）. The median expression levels of CD3ε gene in observation group and mild lead poisoning group （10.22%, 6.08% ） were higher than the control group （P〈0.05）. A significant Positive correlation was found between CD3% CD3ε and seniority in lead poisoning patients.A significant negative correlation was found between CD3ε and blood ZPP, urea δ-ALA （r=-0.358, P〈0.05; r=-0.385, P〈0.05）, but there was no significant correlation between them after controlling for blood lead, urea lead. The expression levels of CD3 genes prove to be a decending order of CD3% CD38, CD3δ in control group, while it was changed for CD3ε, CD3γ, CD3δ in the observation group as well as in mild lead poisoning group. Conclusion Expression of T cell TCR-CD3 complex-mediated gene was changed in lead poisoning patients, it might be related to the body immunodefieiency. The expression level of CD3 ε gene can be used as sensitive immune function screening indicator in Lead poisoning patients.