尿中N-乙酰-β-D-氨基葡萄糖苷酶活力对急性百草枯中毒早期肾损伤的意义

The significance of urine N-acetyl-beta-D-glucosaminidase in kidney injuy with patients acute paraquat poisoning

目的探讨尿中N-乙酰-β-D-氨基葡萄糖苷酶（NAG）活力在急性百草枯中毒早期肾损害中的意义。方法检测44例急性百草枯患者人院后尿中NAG活力并与40例健康对照组进行比较。结果百草枯中毒组尿中NAG活力为（40．7±10．6）U／gCr明显高于健康对照组[（18．6±5．1）U／gCr]，差异有统计学意义（P〈0．01）。NAG活力I〉25U／gCr中毒组30d死亡率（34．4％）明显高于NAG活力〈25U／gcr组（16．7％），两组病死率的差异有统计学意义（P〈0．01）。结论尿中NAG活力≥25U／gCr组百草枯中毒病死率明显升高，尿中NAG活力对急性百草枯中毒早期肾损伤诊断可能有一定意义。

Objectives To test the hypothesis that urine N-acetyl-beta-D-glucosaminidase （NAG） is an early biomarker for acute kidney injury in patients with acute paraquat poisoning. Methods Forty-four patients with paraquat intoxication and 40 age and gender-matched healthy control participants were recruited. The urine N-acetyl-beta-D-glucosaminidase was determined by spectrophotometric methods. Results The urine N- acetyl-beta-D-glucosaminidase activities in the patients with paraquat poisoning were higher than the corresponding values in the control participants （P〈0.01）; The prevalence rate of mortality was significantly higher in subjects with N-acetyl-beta-D-glucosaminidase activities ≥ 25 U/g Cr than in those N-acetyl-beta-D- glucosaminidase activities 〈25 U/g Cr （34.4% vs 16.7%, P〈0.01）. Conclusions The urine N-acetyl-beta-D- glucosaminidase could be used as an early biomarker for acute kidney injury and predictor of mortality in patients with acute paraquat intoxication.