瓜蚜为害对寄主植物主要营养物质和次生物质的影响

Influences of Aphis gossypii Glover feeding on the contents of main nutrients and secondary compounds in host plants

为明确瓜蚜Aphis gossypii Glover与其寄主植物相互作用关系及其机理,本文采用蒽酮比色法、考马斯亮蓝法、磷钼酸-磷钨酸比色法及索氏回流法,分别测定了瓜蚜为害对黄瓜、哈密瓜、南瓜、瓢葫芦和搅瓜等5种寄主植物叶片内可溶性总糖、可溶性蛋白质、单宁及黄酮含量的影响。结果表明,瓜蚜为害后,供试寄主植物叶片中可溶性糖和可溶性蛋白含量显著降低,而单宁和黄酮的含量明显上升。说明瓜蚜为害导致寄主植物营养质量下降,而次生代谢物质含量上升,从而对植食者产生诱导抗性。

In order to investigate the interaction between determined how the aphid feeding affected the contents Aphis gossypii Glover and its host plants, it was of soluble sugar, soluble protein, tannin and flavone in the leaves of its five host plants （Cucumis sativus, Cucumis melo var. saccharinus, Cucurbita moschata var. melonaeformis, Lagenaria siceraria var. turbinate and Cucurbita pepo var. medullosa ） , using anthrone colorimetric method, Coomassie brilliant blue staining, phosphor - molybdenum acid - phosphor- tungstenic acid colorimetry and Soxhlet extraction, respectively. The results showed that the contents of soluble sugar and soluble protein in the leaves of its host plants decreased while the contents of tannin and flavone increased after the host plants were fed by the aphids. This indicated that the aphid feeding caused the decrease of host plant＇s nutrition quality while the increase of harmful secondary metabolite contents, and therefore, induced the resistance to herbivores.