β-磷酸三钙煅烧骨修复兔股骨远端骨缺损

Repairing distal femur defects in rabbits with beta-calcium phosphate sintered bone

背景:自体骨兼具骨引导和骨诱导特性而成为修复骨缺损的金标准,但其来源有限,促使研究人员去寻找各类骨移植替代物。目的:观察β-磷酸三钙煅烧骨作为骨移植替代物修复兔股骨远端骨缺损的效果。方法:制备直径5mm,深12mm的兔股骨远端骨缺损模型,实验侧骨缺损部位植入β-磷酸三钙煅烧骨试件,对照侧仅制造骨缺损模型,不植入任何材料。观察实验动物手术切口局部情况,并制备病理切片,观察材料植入后的骨长入情况。结果与结论:骨缺损部位植入β-磷酸三钙煅烧骨试件后,实验动物的切口愈合良好,在术后4周可以观察到骨缺损周边开始形成新骨,并随着时间的延长新骨形成量逐渐增多,至术后12周时,材料中心部位也可见新骨长入,材料逐渐降解,而对照侧直至术后12周时骨缺损部位仍无新骨长入。结果显示β-磷酸三钙具备良好的成骨性能,是一种优良的骨移植替代物。

BACKGROUND： Autologus bones possess the bone guided activity and osteoinductive properties, becoming the gold standard for repair of bone defects, However, the limitation of resources in autogenous bone causes the research fellows to look for bone graft substitutes.OBJECTIVE： To explore the effectiveness of repairing distal femur defects in rabbit with β-calcium phosphate sintered bone.METHODS： A rabbit model of distal femur defects at diameter of 5 mm and depth of 12 mm was prepared, β-calcium phosphate sintered bone was implanted into the experimental side of the model, and nothing to the control side. Operation incision was observed and the pathological section was prepared to observe bone growth after implantation.RESULTS AND CONCLUSION： The incision healed fine after the operation of implanting the test piece of β-calcium phosphate sintered bone into the defect site in the condyles of femur in rabbits; new bone formation could be observed around bone defects in the rabbits of experimental groups after 4 weeks of the operation, the quantity of the＇new bone increased gradually with the time prolonging, the center of the β-calcium phosphate sintered bone could be found new bone and the materials could be degraded gradually after 12 weeks of the operation. In the other hand, new bone formation could not be found around bone defects until 12 weeks of the operation in the control group, These findings indicate that β-calcium phosphate has a good osteogenesis capacity, and can serve as an excellent bone graft substitute.