明胶联合壳聚糖纤维对磷酸钙骨水泥力学性能的影响

Gelatin combined with chitosan fiber affects the mechanical properties of calcium phosphate cement

背景:已有多种纤维被用于提高磷酸钙骨水泥的强度及抗断裂性能。目的:了解明胶联合壳聚糖纤维对磷酸钙骨水泥力学性能的影响,寻找较为合适的配比。方法:采用2×4析因设计,将质量比为0(蒸馏水),5%的明胶,体积比为0,10%,30%和50%的壳聚糖纤维分别混入磷酸钙骨水泥,检测复合物的抗弯曲强度,扫描电子显微镜观察各组试样断口形态并进行电子能谱分析。结果与结论:各明胶组间抗弯强度差异有非常显著性意义(P<0.001);各体积比纤维间抗弯强度差异有非常显著性意义(P<0.001),其中5%明胶和30%壳聚糖纤维构成的复合物抗弯曲强度最大,达12.31MPa。以蒸馏水为液相的磷酸钙骨水泥固化后,表面可见不规则颗粒,平均微孔直径小于5μm,添加明胶后颗粒似乎黏在一起,微孔直径与前者相似,但是数目少于前者。磷酸钙骨水泥-5%明胶-30%纤维复合物的断口扫描可见拔出纤维的表面黏附有大量颗粒,磷酸钙骨水泥-蒸馏水-30%纤维复合物拔出纤维表面的颗粒明显减少。表明明胶与壳聚糖纤维可提高磷酸钙骨水泥的抗弯曲强度,5%明胶和30%壳聚糖纤维为这种增强模式较为合适的比例。

BACKGROUND： A few of fibers have been used to reinforce the strength and fracture resistance of calcium phosphate cement.OBJECTIVE： To investigate the influence of gelatin combined with chitosan fiber on the mechanical properties of calcium phosphate cement and to look for a more appropriate ratio.METHODS： Comparisons and to look of chitosan fibers at volume fractions of 0, 10%, 30% and 50% together with distilled water or gelatin at mass fraction of 5% respectively in their effects on flexural strength of cement were performed. 2×4 factorial design was adopted, Flexural strength of cement composite fractures was detected, and fractured surface morphology was observed by scanning electron microscope. Energy dispersion analysis was also performed on particles of composites.RESULTS AND CONCLUSION： A significant difference was found between the flexural strength of different gelatin groups and between the flexural strength of different volume fraction groups （P 〈 0.001）. The maximal fiexural strength was obtained when the calcium phosphate cement was reinforced with fiber at volume fraction of 30% and gelatin at mass fraction of 5%, which was 12.31 MPa. With distilled water as the liquid phase, there were irregular particles （mean pore diameter 〈 5 μm） on the surface of the solidified calcium phosphate cement. After gelatin adding, gelatin and particles seemed to stick together, and the mean pore diameter was similar to the above-mentioned, but the number was less than the former. A great amount of particles were seen at the fractured surface of calcium phosphate cement reinforced with fiber at volume fraction of 30% and gelatin at mass fraction of 5%, and the number of particles was decreased significantly at the fractured surface of calcium phosphate cement reinforced with distilled water and fiber at volume fraction of 30%. The mechanical property of calcium phosphate cement could be enhanced by using gelatin and chitosan fiber as two reinforcement agents, and gelatin at the mass fraction of 5% and chitosan fiber at the volume fraction of 30% might be a better ratio for this reinforcement mode.