构建可控释血管内皮生长因子多壁碳纳米管的复合支架

Constructing a vascular endothelial growth factor sustained-released multi-wall carbon nanotube composite scaffold

背景:单纯植入猪小肠黏膜下层修复腹壁缺损存在早期血管化不足,导致修复失败。目的:构建可控释血管内皮生长因子的多壁碳纳米管-猪小肠黏膜下层复合支架,评价其体外控释血管内皮生长因子性能、力学性能及细胞毒性。方法:通过浸染将装载血管内皮生长因子的多壁碳纳米管复合到猪小肠黏膜下层上,构建可控释血管内皮生长因子的复合支架,并根据多壁碳纳米管与猪小肠黏膜下层的不同质量比(0,1%,3%,5%,10%)构建5种多壁碳纳米管-猪小肠黏膜下层。结果与结论:①复合支架的血管内皮生长因子控释性能:随时间的延长,各组的累积浓度增高,且随着多壁碳纳米管质量的增加,释放浓度也逐渐增加。②复合支架的力学性能:1%,3%,5%,10%多壁碳纳米管-猪小肠黏膜下层最大载荷及弹性模量均高于猪小肠黏膜下层(P<0.05),且随着多壁碳纳米管质量的增加,最大载荷逐渐增加。③复合支架的成纤维细胞毒性:多壁碳纳米管在复合支架中质量分数≤5%时对细胞的生长无影响。表明构建的多壁碳纳米管复合支架具备良好的血管内皮生长因子控释性能、力学性能及促进内皮细胞增殖的能力。

BACKGROUND： The implantation of porcine small intestinal submucosa （PSIS） for abdominal wall defects has deficiency in early vascularization, which can result in the failure of abdominal wall reconstruction.OBJECTIVE： To construct a multi-wall carbon nanotube （MWCNT）-PSIS composite scaffold which can sustained-release vascular endothelial growth factor （VEGF）, and to evaluate the VEGF sustained-release performance, mechanical property and cytotoxicity of the composite scaffold in vitro.METHODS： The VEGF-Ioaded MWCNT was integrated with two-layer PSIS by dip dyeing to construct the composite scaffold. According the different weight percents of MWCNT, five groups were divided： PSIS （0%）, 1%,3%, 5%, 10% MWCNT-PSIS.RESULTS AND CONCLUSION： （1） The VEGF sustained-release property of the composite scaffolds： The accumulation concentration of each group increased with the extension of time, meanwhile the released concentration increased gradually with the increasing quality of the MWCNT. （2） The mechanical property of the composite scaffolds： The maximum load and elastic modulus of 1%, 3%, 5%, 10% MWCNT-PSIS groups were superior to original PSIS scaffold （control group） （P 〈 0.05）. The maximum load increased gradually along with the increasing quality of the MWCNT. （3） The fibroblast cytotoxicity of the composite scaffolds： The composite scaffolds that contained ≤ 5% MWCNT were proved to have no significant influence on the fibroblast proliferation. The above results in vitro confirmed the composite scaffold we constructed possesses excellent VEGF sustained-released performance, improves mechanical property and promotes the endothelial cell proliferation.