许旺细胞及小肠黏膜下层复合生长因子缓释微球修复周围神经缺损

Combination of Schwann cells, small intestinal submucosa and growth factor sustained-release microspheres for repair of peripheral nerve defects

背景:前期实验已初步证实许旺细胞复合小肠黏膜下层及碱性成纤维细胞生长因子构建的人工神经具有体外神经活性、趋化性。目的:观察许旺细胞及小肠黏膜下层复合碱性成纤维细胞生长因子缓释微球修复周围神经缺损后神经传导的再通情况。方法:制作SD大鼠坐骨神经缺损模型,随机分组:实验组以许旺细胞及小肠黏膜下层复合碱性成纤维细胞生长因子缓释微球修复,阳性对照组以许旺细胞及小肠黏膜下层复合游离碱性成纤维细胞生长因子修复,阴性对照组以许旺细胞及小肠黏膜下层修复,空白对照组以自体神经修复。结果与结论:术后16周实验组再生神经纤维数目,DiI示踪标记的阳性神经元数量、S-100及神经细丝蛋白的阳性表达率、髓鞘及再生轴突的超微结构恢复、神经传导速度及复合动作电位的改善均优于阳性对照组与阴性对照组(P<0.05)。表明许旺细胞复合小肠黏膜下层及碱性成纤维细胞生长因子缓释微球构建的人工神经可重建坐骨神经缺损后的神经传导通路。

BACKGROUND： To combine Schwann cells （SCs） and small intestinal submucosa （SIS） with basic fibroblast growth factor （bFGF） is a feasible way to construct artificial nerve that possesses nerve activity and chemotaxis in vitro.OBJECTIVE： To observe the recanalization of neural pathways after repairing peripheral defects with bFGF controlled release microspheres combined with SCs and SIS.METHODS： Models of sciatic nerve defects were established in Sprague-Dawley rats, and then randomized into： experimental group repaired with bFGF controlled release microspheres combined with SCs and SIS, positive control group with SCs and SIS combined with free bFGF, negative control group with SCs and SIS, and blank control group with autologous nerve.RESULTS AND CONCLUSION： The number of regenerated nerve fibers, number of Dil tracer-positive neurons, positive expression rate of S-100 and neurofilament protein, ultrastructure of regenerated myelin and axons, nerve conduction velocity and composite action potential in the experimental group were superior to those in the positive and negative control groups at 16 weeks after operation （P 〈 0.05）. These findings indicate that bFGF controlled release microspheres combined with SCs and SIS can reconstruct the neural pathways following sciatic nerve injury.