摘要
探讨丙烯腈对雄性小鼠生殖细胞的毒性作用。 [方法 ]雄性ICR小鼠以 0、2 5、5、10mg/kg体重剂量连续腹腔注射染毒 5d ,2周后处死动物 ,观察睾丸中早期精细胞微核 ;ICR雄性小鼠以 0、6、12、2 4mg/kg体重连续灌胃染毒 5d后于每周末处死部分动物 ,连续 5周 ,分析附睾精子畸形率。 [结果 ]早期精细胞微核实验结果表明 ,染毒剂量≥ 5 0mg/kg ,早期精细胞微核明显增加 ,微核率与染毒剂量之间存在剂量反应关系 (y =0 2 70x- 0 816 ,r=0 988)。精子畸形实验结果表明 ,第 2周起 2 4mg/kg组附睾精子畸形率增加即有显著意义(P <0 0 5 ) ,12、2 4mg/kg体重剂量组染毒后第 5周末附睾精子畸形率极明显升高 (P <0 0 1)。精子畸形种类主要以头部畸形为主 ,主要包括无钩、不定型、香蕉型。 [结论 ]丙烯腈能够引起早期精细胞遗传损伤 ,精子畸形数增加 ,从而影响雄性小鼠的生殖功能。
Objective] This study is to investigate the potential effects on germ cells of male mice exposed to acrylonitrile.[Methods] The rats were given by ip acrylonitrile for 5 days at doses of 0,2 5,5,10mg/kg b.w.to observe the frequencies of micronuclei in early spermatogenic cells after 2 weeks,and by gavage at doses of 0,6,12,24mg/kg b.w.to analyze the rates of abnormal sperms.[Results] The frequencies of micronuclei in early spermatogenic cells were significantly increased at dose≥5 0mg/kg b.w.and there was a distinct dose response relationship ( y=0 270x 0 816,r =0 988).The numbers of abnormal sperms in the epididymis significantly increased at doses of 12 and 24mg/kg b.w.compared with those in control group in the second week after treatment,in 12mg/kg and 24mg/kg groups abnormal sperms highly increased in the fifth week after treatment ( P <0.01).These abnormalities were characterized by sperm heads without a hook or with a blunt hook.[Conclusion] Acrylonitrile could have the mutagenic effect on germ cells and cause increase in numbers of abnormal sperms,so it may affect reproductive function in male mice.
