影响绵羊超数排卵和人工授精效果因素的研究(英文)

Factors Influencing the Superovulation and Artificial Insemination of Sheep

[目的]获得低成本高效率的绵羊超数排卵处理方案和较好人工授精效果的方法。[方法]使用常规超数排卵和人工授精的方法,从可能影响其效果的FSH激素与海绵栓的组合、发情时间间隔、输精次数、公羊个体等4个方面进行研究分析。[结果]海绵栓结合北京产FSH组合的超排效果最差,显著低于其他试验组(P<0.01),使用宁波生产的FSH,结合海绵栓或者CIDR栓,都能获得较好的超排效果;最后一次注射FSH后12h发情的细毛羊供体超排效果最好,显著高于36h组(P<0.01),与0和24h间没有差异;人工输精2次的怀孕率显著高于输精1次(P<0.01);不同公羊个体的受精效果不同。[结论]使用国产FSH和国产海绵栓的组合,能在不影响超排效果的前提下显著降低成本;选择受精效率高的公羊个体,连续输精2次能获得较好的受胎效果。

[Objective] This study aimed to find out a method for low-cost and highly efficient sheep superovulation treatment and artificial insemination. [Method] The factors those probably influencing the results of conventional superovulation and insemination, such as combination of FSH hormone and sponge suppository, estrus interval, number of insemination, and ram individuals were analyzed. [Result] The combination of sponge suppository and FSH produced in Beijing exhibited the poorest effect to superovulation, significantly worse than that of other combinations （P0.01）. The FSH produced in Ningbo, combined with sponge suppository or CIDR produced better effect to superovulation. The superovulation effect was better when the interval from the last FSH injection to estrus was 12 h, significantly better than that when the interval was 36 h （P0.01）; and there was no difference in the superovulation results when the interval was 0, 12 and 24 h. The pregnancy rate of two artificial inseminations was significantly higher than that of only one insemination （P0.01）. Rams themselves had significant influence on fertilization results. [Conclusion] The combination of domestic FSH and domestic sponge suppository cost much less and dose not reduce the superovulation results. Better fertilization result can be obtained if the ewes are inseminated twice with the sperm those gave high pregnancy rate.