β防御素2对大鼠肺部炎症细胞因子的影响

The Impact of rBD2 on the Lung Inflammatory Cytokines of Rat

目的探讨β防御素2(rBD2)对大鼠肺部炎症反应的影响及其可能机制。方法构建大鼠rBD2的cDNA慢病毒载体和RNA干扰载体,双向调节rBD2在大鼠肺组织内的表达水平,ELISA法检测肺组织中IL-1α、IL-1β、IL-4、IL-10的表达变化,Western blot检测肺组织中rBD2的表达,Real time PCR法检测肺组织中rBD2mRNA的表达。结果成功构建rBD2cDNA慢病毒和RNA干扰载体,并成功转染。大鼠肺部感染时,上调rBD2可降低IL-1α、IL-1β等促炎细胞因子的水平,提高抗炎细胞因子IL-4、IL-10的含量(P<0.01)。结论 rBD2可调节大鼠肺组织炎症相关细胞因子的水平,参与并抑制大鼠肺部炎症反应。

Objective To study the impact of rat beta defensin-2（rBD2） on the pulmonary inflammation of rat and the possible mechanism. Methods To establish rat rBD2 cDNA lentiviral vector and vector for rBD2 siRNA. Both upregulation and downregulation of rBD2 expression were conducted to examine its effect on the pulmonary inflammation of rat. ELISA assay was conducted to evaluate the expression of cytokines,including IL-1α, IL-1β, IL-4 and IL-10. The expression of rBD2 in the lung was detected by Western blot; the expression of rBD2 mRNA was detected with Real-time PCR. Results Overexpression and RNAi lentiviral recombinant vectors of rBD2 were successfully constructed. Up regulation of rBD2 expression could decrease the level of IL-1α, IL-1β and increase the level of IL-4,IL-10（P（0.01）. Conclusion rBD2 could regulate the expression of cytokines and play a role as an anti-inflammatory role in the development of pulmonary inflammation in rat.