离子色谱-柱后衍生紫外检测法测定化妆品中碘酸盐与溴酸盐

Determination of Iodate and Bromate in Cosmetics by Ion Chromatography with Postcolumn Deriviation and UV Detection

建立了同时检测化妆品中溴酸盐和碘酸盐的离子色谱-柱后衍生紫外检测器定量分析方法。样品用水提取,经RP柱净化,采用IonPac AS11-HC色谱柱分离,以EG40自动淋洗液发生器生成的KOH为淋洗液梯度洗脱,溴化钾、亚硝酸钠为衍生试剂,二极管阵列检测器检测,外标法定量。结果表明:该方法在2.0～1 000 mg/kg范围内线性关系良好(r≥0.999 8);加标回收率为84%～99%;相对标准偏差小于2%。方法简单、快速、选择性强,可用于化妆品中碘酸盐和溴酸盐的测定。

An ion chromatographic method with post-column deriviation and UV detection for the determination of iodate and bromate in cosmetics was developed and validated. The sample was purified with a guard-column IonPac AG11 - HC （50 mm×4 mm） , and separated on an analytical column IonPac AS11 -HC（250 mm×4 mm） using KOH as an eluent with a self-regeneration suppressor. NaNO2 and KBr were used as the post-column deriviation solution. The analyte was quantitatively analysed by external standard and detected with a DAD detector. The results showed that the calibration curves were linear in the range of 2.0 - 1 000 mg/kg with correlation coefficients not less than 0. 999 8. The spiked recoveries were in the range of 84% - 99% with relative standard deviations （ RSDs, n = 3 ） less than 2% . With advantages of simplicity, good reproducibility and high sensitivity, the method was suitable for the determination of iodate and bromate in cosmetics.