二甲双胍对高糖状态下心肌细胞保护作用的研究

Study on Protective Effect of Metformin on Cardiomyocytes in High Glucose Medium

【目的】探讨二甲双胍对高糖状态下心肌细胞发挥保护作用的机制。【方法】取原代培养24h的心肌细胞，分为6组（均n=15）：对照组（不加任何处理）、高糖组（25mmol／L）、高渗组（甘露醇液）、二甲双胍组（25mmol／L高糖分别加入10mol／L5×10。mol／L、10。mol／L浓度的二甲双胍）。【结果】与对照组比较，高糖组白介素-1B（IL-1p）、白介素-6（IL-6）、B型利钠肽（BNP）、氨基末端脑钠肽前体（NT—proBNP）及casp—sae-3的表达水平明显升高（均P〈0．05）；与高糖组比较，二甲双胍组IL-1p、IL-6、BNP、NT-proBNP及casp—sae-3的表达水平明显降低（均P〈0．05）。【结论】二甲双胍能够抑制炎症因子的表达，减轻高糖引起的心肌损伤。

[Objective]To explore the mechanism of protective effect of metformin on cardiomyocytes in high glucose medium. [Methods] Myocardial cells in primary culture for 24h were divided into control group（ n = 15, without any treatment）, high glucose group（ n ： 15, 25mmol/L）, hypertonic group（ n = 15, manni- tol solution） and metformin group（ n = 15）. The metformin group was given high glucose and supplemented with metformin 10-Smol/L, 5 X 10-5 mol/L and 10 4tool/L, respectively. [Results] Compared with control group, the expression of interleukin-1 beta （IL-113）, interleukin-6 （IL-6）, B-type natriuretic peptide （BNP）, NT-proBNP and caspsae-3 in high glucose group obviously increased（all P （0.05）. Compared with high glu- cose group, the expression of IL-1B, IL-6, BNP, NT-proBNP and caspsae-3 in metformin group obviously de- creased（all P （0.05）. [Conclusion] Metformin can inhibit the expression of inflammatory factors and reduce hyperglycemia-induced cardiomyocyte injury.