HR—HPV持续感染宫颈病变患者细胞免疫状态分析

Analysis of Cellular Immune State of Cervical Lesion Patients with HR-HPV Persistent Infection

【目的】探讨宫颈病变后患者外周血T淋巴细胞亚群CD3、CD4、CD8的表达水平及意义。【方法】选择慢性宫颈炎＋CINI级30例（A组），CINII～HI级30例（B组），宫颈癌30例（C组），用流式细胞仪检测并比较HR_HPV阳性且发生宫颈病变患者外用血T淋巴细胞亚群CD3、CD4、CD8的表达水平。【结果】A组、B组、C组的CD3、CD4水平依次呈下降趋势，而CD8逐渐上升；C组的CD4／CD8比值显著低于A组、B组，并出现倒置现象。A组与B组cD3略下降，但差异无显著性（P〉0．05），余各组间cD3、CD4、CD8、CD4／CD8淋巴细胞的比较均具有差异性（P〈O．05）。【结论】随着宫颈病变程度的加重，机体细胞免疫逐渐发生免疫功能低下，甚至出现免疫抑制状态。

[Objectivel To explore the expression of peripheral blood T- lymphocyte subsets CD3, CD4 and CD8 in patients with cervical lesion and its significance. [Methods] Thirty patients with chronic cervicitis and CINI （group A）, 30 patients with CINII - HI （group B） and 30 patients with cervical cancer（group C） were chosen. Flow cytometry was used to detect and compare the expression of peripheral blood T lymphocyte subsets CD3, CD4 and CD8 in patients with cervical lesion and HR-HPV positive. [ResuhsIFI;om group A, group B to group C, the level of CD3 and CD4 declined by turns, while CD8 raised gradually. CD4/CD8 ratio in group C was signifi- cantly lower than that in group A and group B, and the inversion was observed. CD3 in group A and group B slightly decreased, but there was no significant difference（ P 〉0.05）. There were significant differences in CD3, CD4, CD8 and CD4/CD8 lymphocytes among groups（ P d0.05）. [ConelusionlWith the increasing of the degree of cervical lesion, low immune function of body immune cells occurs gradually, and even immunosuppression state ap- pears.