摘要
目的:通过研究大鼠急性脊髓损伤后单核细胞趋化蛋白-1(MCP-1)的表达及其对巨噬细胞的趋化作用、脑源性神经营养因子(BDNF)的表达变化,探讨脊髓继发性损伤的作用机制及其与损伤后神经修复之间的关系。方法:30只成年Wistar大鼠随机分为各损伤组和假手术组。制备大鼠急性脊髓损伤动物模型,采用免疫组化SABC法,检测损伤后3、6、12、24、72小时MCP-1、BDNF阳性细胞及巨噬细胞的平均光密度值。结果:急性脊髓损伤后3h,损伤区域MCP-1阳性细胞、巨噬细胞、BDNF阳性细胞平均光密度值均比假手术组增加(P<0.05)。MCP-1阳性细胞平均光密度值在24h达峰值(P<0.05),巨噬细胞和BDNF阳性细胞平均光密度值均在72h达到最高(P<0.05)。结论:MCP-1与巨噬细胞参与脊髓继发性损伤,MCP-1对巨噬细胞有趋化作用,巨噬细胞平均光密度值出现的高峰时间滞后于MCP-1阳性细胞;脊髓损伤后BDNF参与神经修复。
Objective： To research the expression of MCP-1 and its effect on macrophages,the expression of BDNF after acute spinal cord injury in rats;To explore the mechanism of spinal cord continual injury and the relationship between it and the restoration of the neural cells. Methods： Thirty Wistar rats were randomly and evenly divided into injury groups and sham-operated group. Prepare the animal model of acute spinal cord injury in rats. The immunohistochemieal SABC method was used to detect the cells expressed MCP- 1,BDNF and the infrkrated macrophages at 3,6,12,24,72 hours separately after the acute spinal cord injury. Results： Three hours after the acute spinal cord injury, the density mean of the positive cells of MCP- 1 BDNF and macrophages were significantly more high than laminectomy groups in damage area （P〈0.05）. The density mean of the positive cells of MCP- 1 was the most high at 24 hours （P〈0.05）. The density mean of macrophages and the positive cells of BDNF were the most high at 72 hours（P〈0.05）. Conclusion： Both MCP-1 and macrophages take park in the spinal cord continual damage.The macrophages can be tended towards by MCP-1.And the peak of the density mean of macrophages was delay the density mean of the positive cells of MCP- 1. After the acute spinal cord injury,BDNF takes park in the restoration of the neural cells.
出处
《甘肃医药》
2013年第3期184-186,共3页
Gansu Medical Journal
