ECM-1和TIMP-2在肝细胞癌患者血清中的表达及其临床意义

Serum levels of extracellular matrix protein 1 and TIMP-2 in patients with hepatocellular carcinoma and its clinical significance

目的通过检测肝细胞癌患者血清中ECM-1和TIMP-2的表达水平,分析ECM-1和TIMP-2与临床病理参数之间的关系及临床意义。方法应用酶联免疫吸附法测定59例肝癌患者血清ECM-1和TIMP-2水平。结果 59例肝癌患者血清ECM-1和TIMP-2平均水平分别为145.08±22.13 pg/ml、30.77±13.89 ng/ml;两者水平均与肿瘤包膜、TNM分级、血管侵犯、肿瘤结节有关(P<0.005),而ECM-1与肿瘤分化程度有关(P<0.005);ECM-1和TIMP-2水平呈显著负相关(r=-0.469,P=0.000)。结论 ECM-1与TIMP-2的表达可能与肝癌侵袭转移密切相关,可作为患者术后复发与转移的预测指标。

Objective To investigate the serum levels of extracellular matrix protein 1 （ ECM-1 ）, tissue inhibitor of matrix metalloproteinase-2 （TIMP-2） in patients with hepatocellular carcinoma （HCC）. Methods Serum levels of ECM-1 and TIMP-2 were detected by enzyme-linked immunosorbent assay in 59 patients with HCC. Results The serum ECM-1 and TIMP-9 levels were 145.08 ± 22. 13 pg/ml and 30. 77 ± 13.89 ng/ml, respectively. And they were significantly associated with tumor capsula, TNM stage, vas- cular invasion and tumor nodes （P 〈 0. 005）. Furthermore, ECM-1 levels were associated with tumor differentiation, which also were positively correlated with TIMP-2 （ r = - 0. 469,P = 0. 000）. Conclusions Expression of ECM-1 and TIMP-2 may be closely related to the invasion and metastasis of HCC, which can be used as a predictor of recurrence and metastasis for postoperative patients.