高效液相色谱法测定鳖甲煎丸中芍药苷和黄芩苷的含量

Determination of Paeoniflorin and Baicalin in Biejiajian Pill by HPLC

目的建立高效液相色谱法测定鳖甲煎丸中的芍药苷和黄芩苷含量。方法采用C18色谱柱,以乙腈-0.05 mol.L-1磷酸二氢钾梯度洗脱,检测波长为230 nm测定芍药苷;以甲醇-水-磷酸(42∶58∶0.5),检测波长为280 nm测定黄芩苷。结果芍药苷和黄芩苷分别在0.076 6～1.532 8μg和0.061 4～0.770 4μg范围内线性关系良好。平均回收率分别为98.0%和96.5%(n=6);RSD分别为0.7%和1.1%。结论建立的此两种活性成分含量测定方法能有效控制该产品的质量。

Objective An HPLC method was established for the determination of paeoniflorin and baicalin in biejiajian pill. Methods Paeoniflorin was separated on a C18 column with gradient elution using the mobile phase of acetonitrile 0.05 mol · L-1 potassium dihydrogen phosphate solution, and detected at the wavelength of 230 nm. Baicalin was separated on the same column with methanol-water-phosphoric acid （42 ： 58 ： 0.5 ） as mobile phase, and detected at the wavelength of 280 nm. Results The calibration curves were linear in the ranges of 0. 076 6-1. 532 8 μg and 0.061 4-0.770 4 μg, the aver- age recovery was 98.0% and 96.5% （ n = 6）, with RSDs of 0.7% and 1. 1%, respectively. Conclusion Two analytical methods were established to determine the two active ingredients for quality control of the biejiajian Pill.