摘要
A method was developed to modify silicon surfaces with good protein resistance and specific cell attachment. A silicon surface was initially deposited using a block copolymer of N-vinylpyrrolidone （NVP） and 2-hydroxyethyl methacrylate （HEMA） （PVP-b-PHEMA） film through surface-initiated atom transfer radical polymerization and then further immobilized using a short arginine-glycine-aspartate （RGD） peptide. Our results demonstrate that the RGD-modified surfaces （Si-RGD） can suppress non-specific adsorption of proteins and induce the adhesion of L929 cells. The Si-RGD surface exhibited higher cell proliferation rates than the unmodified silicon surface. This research established a simple method for the fabrication of dual-functional silicon surface that combines antifouling and cell attachment promotion.
A method was developed to modify silicon surfaces with good protein resistance and specific cell attachment. A silicon surface was initially deposited using a block copolymer of N-vinylpyrrolidone （NVP） and 2-hydroxyethyl methacrylate （HEMA） （PVP-b-PHEMA） film through surface-initiated atom transfer radical polymerization and then further immobilized using a short arginine-glycine-aspartate （RGD） peptide. Our results demonstrate that the RGD-modified surfaces （Si-RGD） can suppress non-specific adsorption of proteins and induce the adhesion of L929 cells. The Si-RGD surface exhibited higher cell proliferation rates than the unmodified silicon surface. This research established a simple method for the fabrication of dual-functional silicon surface that combines antifouling and cell attachment promotion.
基金
financially supported by the National Natural Science Foundation of China (Nos.20974122,21174098)
the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
