摘要
探讨自身抗原ＳＳＡ／Ｒｏ－５２ｋＤ的抗原优势表位，为疾病机制的研究提供依据，根据计算机软件进行的蛋白质序列结构分析。采用ＰＣＲ法克隆自身抗原ＳＳＡ／Ｒｏ－５２ｋＤ多肽片段的ｃＤＮＡ，定向插入表达载体ＰＧＥＸ－５Ｘ－１，并且导入大肠杆菌中表达重组融合蛋白，用ＧＳＴ亲和层析柱进行纯化，经免疫印迹法与病人阳性血清进行反应，结果表明片段Ｒｏ５２３具有较强的抗原性。
The interactions between autoantigen and autoantibody play an important role in the pathogenesis of autoimmune diseases In view of analysis of the epitopes of autoantigen SSA/Ro-52kD to understand the molecular mechanism of autoimmune diseases Based on the sequence analysis of autoantigen SSA/Ro-52kD the six fractions of SSA/Ro-52kD GST-antigen fusion proteins induced by IPTG in E coli JM109 were obtained The epitopes of SSA/ Ro-52kD were analyzed by IBT and competitive inhibition reaction The results show that the epitopes of SSA/Ro- 52kD were existed on a.a.170 270
出处
《基础医学与临床》
CSCD
2000年第5期46-49,共4页
Basic and Clinical Medicine
基金
上海市启明星基金!编号94QB14002
