低硒大鼠不同组织硒蛋白利用硒的优先性

Preference of selenium incorporating into selenoproteins in different tissues in selenium depleted rats

硒（Se） 耗竭20 周的雄性Wistar 大鼠分别饲以含硒为0.01、 0.02、 0.05 和0.20mg/kg 的四种饲料，于1、3、7、14、21 和35 天时宰杀，动态观察不同组织中硒蛋白的变化，了解硒耗竭后补硒时组织及硒蛋白利用硒的优先性，由此推测它们的功能的相对重要性。结果表明硒蛋白利用硒的优先性顺序为硒蛋白P （Se-P）、脱碘酶、磷脂氢谷胱甘肽过氧化物酶（PHGPX）、细胞外谷胱甘肽过氧化物酶（eGPX）、细胞内谷胱甘肽过氧化物酶（cGPX）和硒蛋白W(Se-W)；组织利用硒的优先性顺序首先为脑，其次是甲状腺，睾丸和垂体，最后是心脏、肾脏、肝脏、肌肉、红细胞。

Four different selenium (Se)level diets which contained 0.01 0.02 0.05 0.20 mg/kg diet were fed to male weanling Wistar rats which were depleted of selenium for 20 weeks The rats were killed at 0 1 3 7 14 21 and 35 day of selenium repletion to observe the kinetic change of tissue Se concentration the activities of cellular glutathione peroxidase cGPX extracellular glutathione peroxidase eGPX phospholipid hydroperoxide glu- tathione peroxidase PHGPX type I deiodinase IDI type deiodinase ID and the mRN level of selenoprotein P and selenoprotein W so that we can know the preference of Se incorporating into selenoprotein The results showed that selenoprotein P was expressed in preference to deiodinase the preferential order was followed by PHGPX eGPX cGPX and selenoprotein W during Se repletion and the order for organs was brain thyriod testis pituitary heart kidney liver muscle and red blood cell