检测Ⅳ型胶原蛋白直接双抗夹心法的建立与评价

Establishment and valuation of ELISA for measuring type Ⅳ collagen

目的 :建立血清 型胶原蛋白 (COL - )浓度测定的直接双抗夹心法 ,为临床诊断肝纤维化提供一种新的指标。方法 :采用本科室自制的 COL - 单克隆酶标抗体、多克隆抗体建立的直接双抗夹心法 ,同时采用日本试剂盒测定 6 0份正常和肝脏病人血清 COL- 浓度做对照比较。结果 :两种方法无显著性差异 (P>0 .0 5 ,t=0 .16 6 ) ;两法相关分析 ,γ=0 .834,回归方程为 Y=0 .5 9X+ 70 .874。结论 :直接双抗夹心法测定血清 COL- 操作简便、特异、准确 ,可作为辅助诊断肝纤维化的可靠指标 。

Objective To establish a direct ELISA measurement of serum col Ⅲ and affer a new guideline for liver cirrhosis clinical diagnosis.Method Adopt reserved mono and poly clone antibody of col Ⅳ to establish a direct ELISA measurement.At the same time,we detect the serum col Ⅲ conlentration in 60 normal persons and patients with cirrhosis by using Tapanese reagent case for control.Results There's no remarkable difference between two methods (P>0 05,t=0 166);related analysis:r=0 834,Y=0 59X+70 874.Conclusion The method of direct ELISA measurement of serum col Ⅳ is so simple,precise and specific that is can be the assistant guideline for lover cirrhosis diagnosis.It can be use prevalently.