自体心包瓣“内皮化”的实验研究

Experimental Study on endothelialization of autologous pericardial heart valves in vitro

目的 探索有利于自体心包瓣内皮化的处理方法。方法 将体外培养小牛主动脉内皮细胞接种于不同方法处理的自体牛心包表面。采用3H TdR摄取量测定、扫描电镜及放射免疫法观察不同处理方法对接种后内皮细胞生长、前列环素 (PGI2 )合成量和细胞内cAMP含量的影响。结果 三种不同浓度戊二醛短时间 (10min)处理的心包表面内皮细胞均可生长 ,其中以体积分数为 0 .2 %戊二醛处理组生长情况最好 ,各时间点细胞密度和3H TdR摄取量均高于体积分数为 0 .5 %、0 .6 2 5 %戊二醛处理组 ,而且对内皮细胞功能影响最小 ,不引起细胞内cAMP含量和PGI2 合成量的异常增高。结论 体积分数为 0 .2 %的戊二醛短时间处理心包组织基本无毒性作用 ,可能更利于自体心包瓣术后的内皮化。

Objective To investigate a method of autologous pericardial heart valves (APHV) treatment beneficial to endothelialization. Methods Bovine aortic endothelium cells (BAEC) were cultured and seeded in autologous pericardial pieces treated with different methods in vitro. The influence of different treatment on cellular growing, amount of 3H TdR incorporation and PGI 2 releasing, contents of intracellular cAMP was studied by means of transmission electron microscopy, 3H TdR incorporation experimentation and radioimmunity method. Results The BAEC seeded in bovine pericardial pieces treated with three different concentrations of GA 10 minutes could alive. In the group with method of treatment with 0.2% GA, the growing of BAEC was best, the cellular density and the amount of 3H TdR incorporation at different time spots were higher than that of the other groups and it did not enhance the abnormal synthesis of cAMP and PGI 2 in BAEC. Conclusion The pericardium treated with 0.2% GA 10 minutes has little toxicity to endothelium cell, which indicates that this method is beneficial to endothelialization of APHV postoperatively.