寻常型天疱疮患者一级亲属循环天疱疮抗体的检测分析

Detection of circulating pemphigus vulgaris antibodies in families of patients with pemphigus vulgaris

目的 探讨与寻常型天疱疮 (pemphigusvulgaris,PV)患者可能共享易感基因的亲属中PV抗体的存在情况。方法 对 12例PV患者的 30名一级亲属及 10名正常对照者同时应用免疫印迹及间接免疫荧光 (indirectimmunofluorescence,IIF)方法 ,检测其外周血中循环PV抗体。结果 在 30名亲属中有 19名 (6 3 % )同时用两种方法检出了PV抗体 ,抗体滴度 >1∶16 ,仅有 9名用两种方法检测均为阴性 ,而 10名正常对照者用两种方法检查皆为阴性。结论 在中国人PV患者的一级亲属中高频率地存在着PV抗体的健康携带者。

Objective To detect the autoantibodies on 30 first degree relatives of 12 patients with pemphigus vulgaris (PV), who may share the same susceptible gene with the patients. Methods Immunoblot and indirect immunofluorescence (IIF) method were used to detect circulatory PV antibodies in the peripheral blood. Results PV antibodies were found in 19 of 30 (63%) relatives by both methods, but 10 control serum were all negative by both methods. Conclusion Healthy PV antibody carriers may also exist in high frequency in the first degree relatives of Chinese PV patients.