溴氰菊酯对大鼠脑钙调蛋白含量和蛋白激酶活性的影响

Efffect of Deltamethrin on Calmodulin Content and PKA,PKC Activities in Rat Brain

为探讨溴氰菊酯 (Deltamethrin ,DM )神经毒作用机理 ,采用竞争性固相酶联免疫法 (ELISA)和放射性同位素法分别观察了DM对不同脑区钙调蛋白 (Calmodulin ,CaM )含量和蛋白激酶 (Proteinkinase)活性的影响。结果表明 ,大鼠经DM处理后 ,大脑和小脑CaM含量无明显变化 ,而大脑皮层和海马中PKA ,PKC活性明显增强 ,同时 ,脑细胞膜和细胞浆中PKC活性亦明显升高。说明蛋白激酶活性增高使脑内蛋白质磷酸化作用增强可能是DM神经毒作用机理之一。

To make further understanding the mechanism of the neutotoxicocity of Deltamethrin (DM),Calmodulin(CaM) content and PKA,PKC activities were determined in different brain regions.The result illustrated;No obvious change of CaM content was observed in cerebral and cerebellum after DM treatment.The activities of PKA and PKC were significantly induced by DM.Activities of PKC in membrance and cytosol were also increased by DM.All the results demonstrated that the phosphorylation of brain proteins by PKA and PKC may by one of the mechanisms of the neurotoxicity of DM.