盐酸氟桂利嗪对鼠肝细胞钙超载的作用

Effects of Flunarizine on Calcium Distribution of Hepatic Ischemic/Reperfusion Injury in Rats

目的 :观察再灌注损伤时盐酸氟桂利嗪 (flunarizine,FL U)对肝组织及肝细胞线粒体钙超载的影响。方法 :Wistar大鼠随机分为 3组 ,每组 13只。A组为对照组 ,B组为缺血 /再灌注组 ,C组为 FL U组 ,其中 B、C两组均阻断肝门造成肝脏完全缺血 30 m in后再灌注 90 m in。测定各组动物血清中 AL T、L DH、TBI含量、肝组织及肝细胞线粒体钙含量 ,行线粒体超微结构观察。结果 :与 A组比较 ,B组血清 AL T、L DH活性显著增加 (P <0 .0 1) ,肝组织及肝细胞线粒体钙含量显著增加 (P <0 .0 1) ,肝细胞线粒体呈损伤性改变 ;与 B组比较 ,C组预用 EL U后血清AL T、L DH活性增高程度降低 (P <0 .0 1) ,肝细胞线粒体超微结构病理损伤程度减轻 ,肝组织及肝细胞线粒体钙含量降低 (P <0 .0 5 )。结论 :钙向组织和细胞内转移是肝脏缺血 /再灌注损伤的原因和重要特征之一 ,预用 FL U可减少再灌注时肝细胞钙超载程度 ,保护肝细胞线粒体 ,对肝脏缺血 /再灌注损伤有一定的保护作用。

Objective:The study was to investigate the effects of pretreatment with flunarizine(FLU) on calcium of liver cells associated with hepatic ischemia and reperfusion. Methods:Thirty nine rats were randomized into 3 groups (13 in each group).Group A was control. Group B was processed in a sham operation. Group C was pretreated with flunarizine(20 mg/kg). Results:Ninety minutes of hepatic reperfusion after half an hour of complete ischemia significantly increased in the level of ALT and LDH in serum (P<0.01),calcium in liver tissue and mitochondrial(P<0.01) Flunarizine could suppress the elevation of ALT and LDH in serum of rats (P< 0.01 ), and decrease the contents of calcium(P<0.05)during the period of reperfusions after ischemia.Ultrastructural examinations showed damages of mitochondria. Conclusion:Calcium overload may play an important role during hepatic ischemia/reperfusion injury .Pretreatment with flunarizine has protective effects on liver injury caused by hepatic ischemia/reperfusion.