头穴丛刺对慢性脑缺血大鼠学习记忆能力及TH表达的影响

Effects of Scalp Cluster-needling on Learning and Memory Ability and the Expression of TH in Chronic Cerebral Ischemic Rats

目的:研究头穴丛刺对慢性脑缺血大鼠的学习记忆能力及相关蛋白表达的影响。方法:采用Sarti等改良的2VO即分次结扎双侧颈总动脉术制作慢性脑缺血大鼠模型,通过Morris水迷宫、光镜、免疫组化等方法观察头穴丛刺对慢性脑供血不足模型大鼠的影响。结果:头穴组大鼠水迷宫潜伏期明显短于模型组(P<0.05);光镜下头穴组大鼠海马CA1区及额叶皮层细胞形态明显优于模型组(P<0.05);免疫组化检查酪氨酸羟化酶(TH)的表达较模型组明显上调(P<0.05)。结论:头穴丛刺可明显的改善慢性脑缺血大鼠学习记忆能力,其机制可能与上调TH的表达有关。

Objective： In order to study the effects on the learning and memory ability and the expression of TH of scalp cluster - needling of chronic cerebral ischemic rats. To explore a new method that will be good to treat the chronic cerebral ischemic patients in clinic. Methods ：Sarti＇s 2VO was used to make the chronic cerebral ischemic rats. Morris water maze was used to evaluate the effects of scalp cluster - needling on chronic cerebral ischemic rats in their learning and memory abilities ; HE was used to evaluate the effects of scalp cluster - needling on tapes of neural ceils of chronic cerebral ischemic rats; immunohistochemical test was used to evaluate the effects of scalp cluster - needling on the expression of TH of chronic cerebral ischemic rats. The SPSS17.0 Statistics software was used to analyze data of variance. Results：Expression of TH was increased significantly （P 〈0.05）and the rats＇learning and memory ability were improved a lot in the scalp -cluster group and the medicine group, compared with those in the sham operation group（ P 〈 0.05 ）. There was a significant difference （ P 〈 0.05 ）. Conclusion： Scalp cluster - needling can improve the learning and memory ability of the chronic cerebral ischemic rats. The mechanism probably related to that scalp cluster -needling can improve the expression of TH.