桃红四物丸促兔自体皮片移植术后不同时限VEGF表达的研究

Effect of Taohong Siwu pills on expression of vascular endothelial growth factor at different time points after skin autograft transplantation in rabbits

目的:研究桃红四物丸对兔自体厚中厚皮片移植术后3个不同时限皮片成活的影响。方法:将60只新西兰大白兔随机分为两组,均进行皮片移植手术造模,治疗组予桃红四物丸灌胃治疗,对照组予等容积蒸馏水灌胃。通过免疫组化染色的方法,观察两组术后5d、8d和14d皮片VEGF的表达情况,并进行比较。结果:治疗组在术后5d、8d和14d均有较高的VEGF积分光密度值,其在3个时限的值均高于对照组(P<0.05)。两组内3个时限比较,VEGF的积分光密度值在术后14d达最高值(P<0.05)。结论:桃红四物丸对兔自体厚中厚皮片移植术后皮片VEGF的表达有促进作用,且在术后14d的作用最明显。

Objective：To investigate the effects of Taohong Siwu pills on the survival of thick split-thickness skin autografts at three time points after transplantation in rabbits.Methods：Sixty New Zealand white rabbits were randomly divided into treatment group and control group to receive skin autograft transplantation.The treatment group was given Taohong Siwu pills.Immunohistochemical assay was used to observe the expression of vascular endothelial growth factor（VEGF） in skin autograts at 5,8,and 14 days after operation.Results：Compared with the control group,the treatment group had significantly higher integrated optical densities（IODs） of VEGF at 5,8,and 14 days after operation（P〈0.05）;the IOD reached the peak level at 14 days in both groups（P〈0.05）.Conclusion：Taohong Siwu pills can promote the expression of VEGF in thick split-thickness skin autograft after transplantation in rabbits,with the most marked effect at 14 days after operation.