头孢氨苄与相似化合物头孢羟氨苄的拉曼光谱比较

Comparison of Raman Spectrum of Cefalexin and Its Similitude Cefadroxil

目的:比较分析头孢氨苄及其相似化合物头孢羟氨苄的拉曼光谱,对结构特征相关性进行分析和探讨。方法:采用傅里叶拉曼光谱仪、显微拉曼光谱仪及便携式拉曼光谱仪对头孢氨苄进行光谱检测,并对相似化合物头孢羟氨苄进行了傅立叶拉曼光谱检测。结果:在3台不同的拉曼仪的3种不同的激发光光源的激发下,头孢氨苄的大部分特征峰的峰位基本一致,但相对峰强却有区别;头孢氨苄与头孢羟氨苄的傅立叶拉曼光谱存在一定的差异,体现了化学结构差异与拉曼光谱差异的相关性。结论:拉曼光谱相对峰强的差异反映了激发光对不同基团或骨架结构的选择性激发。该法简单、快速、可靠、专属性强,可实现自动识别,对样品无损,有望成为药品日常监督中一种有效的鉴别手段。

Objective：To compare the differences of Raman spectrum of cefalexin and its similitude cefadroxil to explore the structural property relationship.Method：Cefalexin was detected using a Fourier Raman spectrometer,a micro-Raman spectrometer and a portable Raman spectrometer,and cefadroxil was studied using a Fourier Raman spectrometer.Result：The major characteristic peaks of cefalexin were basically consistent under the three different excitation light sources in the three different Raman spectrometer,while the relative intensity of the characteristic peaks showed difference.There were differences in the Fourier Raman spectrum of cephalexin and cefadroxil,suggesting the correlation of chemical structure and Raman spectrum.Conclusion：The differences in the relative intensity of Raman characteristic peaks reflect the selective excitation of the source to different groups and skeleton structure.The method is reliable,rapid,accurate and specific with automatic identification and without sample damage,and may be an effective identification method in the daily inspection.