人结直肠癌组织中肿瘤相关巨噬细胞的检测

Detection of tumor-associated macrophages in colorectal cancer tissue

目的:肿瘤相关巨噬细胞(TAMs)在人结直肠癌组织中的定量与分布检测。方法:采用Real-timePCR对30例人结直肠癌手术切除标本及15例内镜取材的正常结直肠标本中TAMs标记物CD68mRNA表达水平进行定量检测,应用免疫组织化学方法检测标本中TAMs的组织分布。分析结直肠癌手术切除标本中CD68mRNA的表达与临床病理参数之间的关系。结果:CD68mRNA在结直肠癌组织中的表达高于正常结直肠组织(t=2.720,P<0.001)。不同临床病理参数的结直肠癌组织中CD68mRNA水平差异无统计学意义(P>0.05)。TAMs分布于结直肠癌组织间质和肿瘤上皮细胞中。结论:TAMs高水平分布于结直肠癌组织间质和肿瘤上皮细胞中。

Aim：To detect the level of tumor-associated macrophages（TAMs）in human colorectal cancer（CRC）tissues.Methods：The expression level of TAMs marker CD68 mRNA was quantified by Real-time PCR in 30 cases of human surgical specimens from CRC and 15 cases of normal colorectal tissue from endoscope.The relationship between the expression of CD68 mRNA and clinicopathological parameters was analyzed in CRC tissue.The distribution of TAMs in CRC tissue was confirmed with the CD68 monoclonal antibody staining.Results：The expression level of CD68 mRNA in CRC tissues was higher than those in normal colorectal tissue（t=2.720,P0.001）.CD68 mRNA level in CRC tissue from different clinlica pathological parameters was not significant（P0.05）.TAMs distributed in the stroma of the tumor tissue and the epithelium cells of CRC tissue.Conclusion：There are more TAMs in stroma tissue and epithelium cells of CRC.