醉椒素脂质体的制备及其理化性质考察

Study on Preparation of Kavain Lipsomes and Chemicophysical Propery

制备醉椒素脂质体并对其稳定性进行考察.采用薄膜—超声分散法制备脂质体,利用高效液相色谱法测定其主药含量,并考察其粒径、Zeta电位、包封率等指标及在室温与4℃保存14 d的稳定性.所得脂质体粒径小而均匀,粒径均值为(161±5)nm,Zeta电位为(2.37±0.2)mV,包封率均在77.09%以上,稳定性考察各项指标均无明显改变.由此可以得出结论,所制制剂包封率较高,稳定性良好.

Kavain lipsomes were prepared and the chemicophysical propery was studied. Kavain lipsomes were prepared by dispersion film-ultrasonication method and the content of kavain was determined by HPLC. The character and partical size of kavain lipsomes were observed. The lipsomes were regular under its microphotograph and the size range was （161 ± 5）nm. The surface charge of lipsomes was （2.37 ± 0.2） mV. The encapsulation efficiency of kavain in lipsomes was 77.09%. No obvious changes were found in the above indexes. The kavain liposome prepared by dispersion film-ultrasonication method was of high encapsulation efficiency and of good stability.