产抗生素南极海洋放线菌的筛选及培养条件优化

Screening and Optimization of Culture Conditions of Antarctic Marine Actinomycete Producing Antibiotics

对32株南极海洋放线菌进行抑菌活性实验,筛选出3株具有一定抑菌活性的放线菌,分别命名为GD-F1、GD-F2和GD-F3,其中GD-F2的抑菌活性较高;探讨了碳源、氮源、pH值等条件对GD-F2产抗生素的影响,确定GD-F2产抗生素的最佳培养条件为:碳源为可溶性淀粉、氮源为大豆粉或酵母膏、用天然海水和蒸馏水各一半配制培养基、无机盐浓度为改良高氏一号培养基的一半、pH值为7.0,为南极海洋放线菌的机理研究和应用奠定了基础。

The antibiotic activity of 32 strains of antarctic marine Actinomycete were tested and 3 strains GD-F1 ,GD-F2,GD-F3 with antibiotic activity Were screened,among which strain GD-F2 had the highest antibi-otic activity. The culture conditions for strain GD-F2 were obtained as follows： with soluble starch as carbon source,with bean powder or yeast extract as nitrogen source,the medium with pH value of 7.0 was made with natural sea water-distilled water（1 ： 1）, the concentrations of inorganic salts were half of those of improved Gao No. 1 culture medium. This study will lay the foundation for adaptation mechanism research and biotechnologi-cal application of antarctic marine Actinomycete.