硒对大鼠腺胃癌的作用及其对腺胃、胰CGRP阳性细胞的影响

EFFECTS OF SELENIUM ON GLANDULAR STOMACH CARCINOMA AND ITS INFLUENCE ON GLANDULAR STOMACH AND PANCREAS POSITIVE CGRP CELLS IN RATS

目的：探讨硒在机体自然抗肿瘤发生过程中的作用。方法：用 ＭＮＮＧ（２０ ｍｇ/ｋｇ）灌胃诱导大鼠腺胃粘膜异倍体形成（大鼠腺胃癌模型形成）。用流式细胞仪测定硒对大鼠腺胃幽门粘膜异倍体形成的影响，用免疫组织化学ＡＢＣ法观察硒在大鼠腺胃粘膜异倍体形成过程中，大鼠腺胃、胰ＣＧＲＰ阳性细胞的变化。并对以上结果进行图像分析及统计学处理。结果：（１）硒能抑制ＭＮＮＧ所致大鼠腺胃粘膜细胞异倍体的形成。（２）在ＭＮＮＧ所致大鼠异倍体的形成过程中，其腺胃、胰ＣＧＲＰ阳性细胞的免疫组织化学反应与正常组大鼠相比较均增强（Ｐ＜０．０５，Ｐ＜０。０１）；而在加硒饲料组大鼠腺胃、胰中 ＣＧＲＰ阳性细胞的免疫组织化学反应与正常组大鼠相比较无显著性差异，但与实验对照组大鼠相比较则分别减弱（Ｐ＞０．０５）和明显减弱（Ｐ＜０．０１）。结论：硒和ＣＧＲＰ阳性细胞可能参与了预防和抑制ＭＮＮＧ所致胃粘膜细胞异倍体的形成。

Objective：To investigate the role of dietary selenium in natural carcinogenesis-resistantance. Methods: In the experiment, Wistar rats stomach carcinogenesis (formation of aneuploid cells in the glandular stomach mucosa) was induced by MNNG (N-methyl-N-nitroso-guanidine, 20 mg/kg) gavage. The inhibitory effect of dietary selenium on incidence of aneuploid cells in mucosal epithelium of gastric antrum was determined by flow cytometry (FCM). Immunohistochemical changes of positive CGRP cells in Wistar rats glandular stomach and pancreas were detected by immunohistochemical ABC method in the course of selenium preventing gastric carcinogenesis. Image analysis and statistical analysis were carrived out with all results above. Results: (1)Dietary selenium might inhibit the formation of aneuploid cells in Wistar rats glandular stomach. (2)The immunohistochemical reaction of Wistar rat glandular stomach and pancreas positive CGRP cells were both significantly stronger in experimental than in nonmal control group in the course of the rat stomach carcinogenesis(p<0. 05,P<0. 01). The immunohistochemical reaction of Wistar rat glandular stomach and pancreas positive CGRP cells had no significant difference in selenium diet group compared with that in normal control group. But slightly weaker and significantly weaker respectively in selenium diet group than that in experimental control group. Conclusion: Selenium and CGRP positive cells might be involved in the prevention and inhibition of the formation of aneuploid cells in the glandular stomach mucosa induced by MNNG gavage.