摘要
目的：观察脑缺血及缺血再灌流海马ＣＡ１区 ５－ＨＴ的变化，探讨脑缺血海马ＣＡ１区神经元损伤与 ５－ＨＴ变化的关系。方法：用免疫组织化学ＡＢＣ法及图像分析技术对沙土鼠脑缺血海马ＣＡ１区５－ＨＴ含量变化进行研究。结果：（１）脑缺血１０ ｍｉｎ时，沙土鼠海马ＣＡ１区５－ＨＴ免疫反应阳性纤维的平均光密度值（ＯＤ）与对照组比较无显著性差异，而缺血 ３０ ｍｉｎ时，ＯＤ值则下降，４ ｈ时下降得更多，Ｐ＜０． ０５。 （２）脑缺血 １０ ｍｉｎ再灌流３０ ｍｉｎ时 ５－ＨＴ的 ＯＤ值即降低，再灌流 ４ ｈ时进一步降低，再灌流 ２４ ｈ时最低，Ｐ＜０．０５，至 ３６ ｈ时ＯＤ值逐渐恢复正常。结论：沙土鼠海马ＣＡ１区 ５－ＨＴ含量在脑缺血及缺血再灌流时均降低，提示海马ＣＡ１区神经元缺血损伤可能与 ５－ＨＴ的紊乱有关。
Objective：To observe the change of 5-HT in CAI sector of hippocampus in gerbils following cerebral ischemia and reperfusion for discussing the relationship between the damage of neurons in CA1 sector and the change of 5-HT after cerebral ischemia. Methods: The change of 5-HT in CA1 sector of hippocampus in gerbils following cerebral ischemia and reperfusion was examined by ABC method of immunohistochemistry and image analysis technique. Results: (1) The mean value of optic density (OD) of 5-HT immunoreaction fiber in CA1 sector had no significant difference between the 10 min ischemic treated group and control group, whereas the OD of 5-HT was decreased significantly in 30 min and 4 h ischemia treated group. (2) The OD of 5-HT immunoreaction fiber in CA1 was decreased in 10 min cerebral ischemia following 30 min and 4 h reperfusion and was the lowest after 24 h reperfusion. After 36 h reperfusion, the OD became normal gradually. Conclusion: The content of 5-HT in CA1 sector of hippocampus is decreased in cerebral ischemia and reperfusion. The change of 5-HT may be involved in neuron damage of CA1 in cerebral ischemia and reperfusion.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2000年第5期428-431,共4页
Chinese Journal of Anatomy
