酶法制备明胶提胶前处理条件

Study on Pretreatment Processing of Gelatin Extraction from Tilapia Bones

作者采用酶法制备明胶。以罗非鱼骨为提取明胶的原料,优化了鱼骨的盐酸脱盐工艺和脱盐骨素利用木瓜蛋白酶酶解工艺。研究结果表明,盐酸脱盐条件为:鱼骨用0.6 mol/L盐酸脱盐,液料比为5 mL/g,浸酸时间为6 h,期间每隔1.5 h换酸一次;木瓜蛋白酶预处理工艺最优条件为:温度为46.5℃,pH 5.3,反应时间为72 min,液料比为3 mL/g,酶的添加量为2 480 U/g(鱼骨)。经过提胶,得到了质量和产率都较高的明胶产品。酶法制取明胶减少了工业废水的产生,缩短了生产周期,并为罗非鱼的综合利用提供了新途径。

This paper is designed to extract gelatin from tilapia bone with the method of Enzymatic Hydrolysis.The effect of demineralizing and hydrolyzing conditions on extraction were analysed.In this research we can show the demineralizing condition is tilapia bone added to 0.6mol/L HCl with solution-to-bone ratio 5 mL/g for 6 h and change the acid solution every 1.5 h.the optimal conditions of enzymolysis is the temperature 46.5 ℃,pH 5.3,time72 min,the protease solution-tobone ratio was 3 mL/g,amount of enzyme 2480 U/g（bones）.