大鼠大脑内血管的氮能神经支配

NITERGIC INNERVATION OF THE CEREBROVASCULATURE IN THE RAT BRAIN

目的：观察大鼠大脑内氮能神经元及血管的氮能神经支配，阐述一氧化氮在脑内血管中的作用。方法：大鼠经灌流固定后用恒冷切片机漂浮切片，还原型尼克酰胺腺嘌呤二核苷酸脱氢酶（ＮＡＤＰ－ｄ）染色。结果：氮能神经无依形态分为双极和多极，见纤细的树突与血管紧密相连及串珠样血管周围神经丛。结论：大脑ＮＡＤＰＨ－ｄ阳性神经元纤细的树突与脑内血管密切相关，提示一氧化氮在调控脑血管张力和血液循环方面有重要作用。

Objective：To observe the nitergic innervation of blood vessels and nitergic neuron in the rat brain, and further prove the effect of nitric oxide in the blood vessels. Methods: Brain were ,removed from 5 Wistar rats by perfusion fixation. Sections were cut by a freezing-microtome, freefloating sections were stained for NADPH-d histochemical method. Results: Nitergic neurons were detected in the rat brain, 2 types could be readily distinguished: bipolar neurons and mutipolar neurons. Their fine branching dendrities closely connected blood vessels. We found fine perivascular fibers of varicose NADPH-d positive fibers plexus also in closely associated with microvessels. Conclusion: NADPH-d positive neurons scatter and their fine dendrites is closely associated with blood vessels. This provide anatomical basis for the innervation of the vessels by nitrergic nerve fibers, suggesting that NO involves in regulation of vascular tone of the brain blood vessels and cerebral circulation.