抑癌基因PTEN在原发性乳腺浸润性导管癌中的表达及临床意义

Expression and clinical significance of PTEN gene in breast cancer

目的研究乳癌PTEN蛋白表达水平与临床病理因素的关系,探讨其临床意义。方法检测40例乳癌组织、40例癌旁组织和20例乳腺囊性增生病PTEN蛋白表达水平(免疫组织化学SP法)。结果 PTEN蛋白高表达,乳癌组织9例、癌旁组织22例、乳腺囊性增生病19例,3组差异有统计学意义(P<0.01)。对于PTEN蛋白表达水平,乳癌组织学级别高者显著高于级别低者,腋淋巴结阳性者显著低于阴性者,雌激素阳性者显著高于阴性者(均P<0.05)。PTEN蛋白表达水平与年龄、肿瘤大小、孕激素水平、Cerb-2水平和临床分期无关,但随年龄增长和肿瘤增大,呈下降趋势。结论 PTEN低下或丢失与乳腺的癌变有关,乳癌组织PTEN蛋白失表达与组织学分级、腋淋巴结转移及雌激素水平密切相关。

[Objectives] To investigate the correlation between PTEN expression level of breast cancer and clinical pathological factors, and explore its clinical significance. [Methods] To detect expression level of PTEN in 40 cases of breast cancer tissues, 40 cases of adjacent tissues and 20 cases of hyperplasia of mam- mary gland （Immunohistoehemical SP method）. [Results] PTEN expression was positive in 9 cases of breast cancer tissues, 22 cases of adjacent tissues, 19 cases of hyperplasia of mammary gland, There were significant differences in the three groups （P〈0.01）. For PTEN level, the high- level tissue of breast cancer was signifi- cantly lower than those of low-level, positive axillary lymph nodes significantly lower than negative ones, es- trogen-positive significantly higher than negative ones all （P〈0.05）. PTEN level was not related to age, tumor size,progesterone level, Cerb-2 and TNM stage, but declined with the increasing of age and tumor size. [ Conclusion] Low level or loss expression of PTEN has relation to the process of breast cancer, the loss ex- pression of PTEN in breast cancer is closely associated with axillary lymph node metastasis, histological grade and progesterone level.