乙型肝炎病毒基因分型与基因变异的临床相关性分析

Analysis of HBV genotypes and gene mutations

目的探讨乙型肝炎病毒(HBV)基因分型与基因变异的关系。方法采用基因测序法检测102例HBV感染者血清标本,用特异的引物对待检标本HBV P区进行全序列测定,对测序结果分析有无突变产生和基因分型。结果 102例HBV感染者标本中基因型分布比例:B基因型12例,阳性率为11.8%;C基因型89例,阳性率为87.3%;D基因型1例,阳性率为0.9%。P区基因序列突变结果显示,102例标本中发生突变的有84例,其中YIDD 50例,占59.5%;YVDD 24例,占28.6%;181V 9例,占10.7%;202G 1例,占1.2%。HBV B、C两基因型间HBV基因变异类型差异无统计学意义。结论①天津地区流行的HBV基因型主要是B型和C型,其中C型为优势基因型。②本地区HBV P区基因序列突变与基因分型无明显关系。

[Objective] To investigate the relation between HBV genetypes and P gene mutation. [Meth- ods] Using gene sequencing technology, 102 HBV infected serum samples were detected. HBV mutations were identified by DNA Star compared with standard sequence. Genetypes were analyzed by viral genetyping tool. [Results] HBV genetyping showed that 12 HBV type B patients, 89 HBV type C patients and 1 HBV type D. Rate accounted for 11.8%, 87.3% and 1% respectively of all the participants. P gene mutation results showed that 84 specimens were mutated in 102 cases, including 50 cases of YIDD, 59.5%, 24 of YVDD, 28.6%, 9 of 181V, 10.7%, 1 of 202G, 1.2%. [Conclusions] The popular HBV genotypes in Tianjin are genotype B and genotype C, type C is the major genotype. HBV P mutations and genotypes have no signifi- cant difference in Tianjin.