意向性运动疗法对MCAO模型脑梗死体积的影响

Effect of willed movement therapy on the cerebral infarct volume of MCAO model

目的构建局灶性脑缺血大鼠意向性运动治疗模型,确定意向性运动疗法对行为学及脑梗死体积的影响。方法建立MCAO模型,随机分为WM(意向性运动组)、EM(环境改变组)、CR(一般康复组)、MCAO(普通对照组)。诱导WM组进行意向性运动,测量爬梯频率、神经功能评分、前后肢抓握功能三方面行为学指标,并通过TTC染色计算其脑梗死体积。结果与MCAO组相比,WM、EM、CR组梗死体积明显减小(P<0.05)。与EM组相比,WM组梗死体积明显减小(P=0.003<0.05);与CR组相比,WM组梗死体积明显减小(P=0.041<0.05);功能评分与梗死面积正相关、爬梯次数与梗死面积负相关。结论康复训练可明显减小脑梗死体积,意向性运动疗法与一般的康复锻炼相比具有更显著的效果。

[ Objective ] To identify the effects of willed movement therapy on the behavior and cerebral infarction volume, we construct the focal cerebral ischemia rat model treated by willed movement therapy. [Methods] We established Middle Cerebral Artery Occlusion （MCAO） model, and model rats were randomly divided into four groups： WM （willed movement group）, and EM （environmental modification group）, and CR （common rehabilitation group）, and MCAO （general controlled group）. The rats of WM group were induced to do willed movement, then we measured their three behavioral indicators, including the frequency of climbing, scoring of neural function and ability of limbs＂ grabbing. Finally we calculated their cerebral infarction volume by （TTC staining method. [Results ] Compared with MCAO group, cerebral infarction volume of WM, EM and CR group significantly reduced （P 〈0.05）. Compared with EM group, cerebral infarction volume of WM group significantly reduced （P =0.003〈0.05）; Compared with the CR group, cerebral infarction volume of WM group significantly reduced （P =0.041 〈0.05）; Functional scoring was positively correlated with cerebral infarction volume and frequency of climbing was negatively correlated with it. [Conclusions] Rehabilitation training may significantly decrease the cerebral infarction volume. In comparison to general physical therapy, willed movement therapy has a more significantly positive effect.