期刊文献+

性控精液在牛体外性控胚胎生产中的应用

Application of Sexed Semen on the in vitro Bovine Sexed Embryos
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摘要 利用性控精液结合体外胚胎生产技术是获得预知性别后代的一种有潜在效率的方法。自20世纪90年代,利用流式细胞仪将X精子和Y精子分离开来,使畜牧生产性别选择的梦想变为现实,性控精液得到了极大的推广应用。本文重点阐述利用性控精液进行胚胎体外生产的研究和生产状况。 Use of sexed semen in conjunction with in vitro embryo production is a potentially efficient means of obtaining offspring of predetermined sex. The ability to sort individual sperm cells into viable X- and Y-chromosome-bearing fractions made producers' sex selection dreams reality in the 1990s and now semen can be sexed with greater than 90% accuracy with use of a flow cytometric cell sorter. However, there are some drawbacks to implementing the extensive use of sexed semen technology include the apparent lower fertility of sorted sperm, the lower survival of sorted sperm after cryopreservation and the reduced number of sperm that could be separated in a specified time period, The issues of In vitro production of bovine embryos using sexed semen and its application prospect are discussed in this review.
出处 《中国奶牛》 2013年第4期28-30,共3页 China Dairy Cattle
基金 北京市奶牛良种选育与繁育体系建设。项目(D12110500330000)
关键词 性控精液 胚胎 Bovine Sexed semen Embryo
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参考文献21

  • 1Hohenboken WI). Applications of sexed semen in cattle production[J]. Theriogenology 1999;52:1421-33.
  • 2Weigel KA. Exploring the role of sexed semen in dairy production systems[J]. J Dairy Sci 2004;87(Suppl E):E120-30.
  • 3Amaun RP. Issues affecting commercialization of sexed sperm[J]. Theriogenology 1999;52:1441-57.
  • 4Foote P.H. Review: dairy cattle reproductive physiology research and management-past progress and future prospect[J]. J Dairy Sci 1996;79:980-90.
  • 5Johnson LA, Welch GP., P.ens W. The Beltsville sperm sexing technology: high-speed sperm sorting gives improved sperm output for in vitro fertilization and Al[J]. J Anita Sci 1999;77(Suppl 2):213-20.
  • 6Johnson LA. Sexing mammalian sperm for production ofoffspring:the state-of-the-art[J]. Anim Reprod Sci,2000,60/61:93-107.
  • 7Garner DL. Sex-sorting mammalian sperm: concept to application in animals[J]. J Androl 2001,22:519-26.
  • 8Hasler JF, Henderson WB, Hurtgen PJ, et al. Production, freezing and transfer of bovine IVF embryos and subsequent calving results[J]. Theriogenology 1995,43:141-52.
  • 9Hunter RHF. Advances in deep uterine insemination: a fruitful way forward to exploit new speml technologies in cattle[J]. Anita tteprod Sci 2003,79:157-70.
  • 10Morrell JM. Applications of flow cytometry to artificial insemination: a review[J]. Vet P.ec 1991,129:375-8.

二级参考文献23

  • 1Johnson LA, Flook JP & Hawk HW. Sex preselection in rabbits: live birth from X and Y sperm separated by DNA and cell sorting [J]. Biology of Reproduction 1989; 41:199-203.
  • 2Cran DG, Johnson LA, Miller NG, Cochrane D & Polge CE. Production of bovine calves following separation of x and y chromosome bearing sperm and in vitro fertilization[J].Veterinary Record 1993;132:40--41.
  • 3Johnson LA. Sex preselection in swine: Altered sex ratios in offspring following surgical insemination of flow sorted X and Y bearing sperm[J].Reproduction in Domestic Animals 1991;26: 195-204.
  • 4Hamano K, Li X, Qian X. Gender preselection in cattle with intracytoplasmica injected, flow cytometrically sorted sperm heads.[J].Biol Reprod, 1999, 60:1194-1197.
  • 5Seidel GE & Garner DL.Current status of sexing mammalian spermatozoa[J]. Reproduction 2002; 124:733-743.
  • 6Li XH, Hamano K, X. Qian, K. Funauchi, M. Furudate and Y. Minato.Oocyte activation and parthenogenetic development of bovine oocytes following intracytoplasmic sperm injection [J]. Zygote, 1999, 7 233-237.
  • 7Lu K H, Cran D G, Seidel G E. In vitro fertilization with flow-cytometrically-sorted bovine sperm.[J].Theriogenology, 1999, 52:1393-1400.
  • 8Lu K H, Suh T K, Seidel G E. In vitro fertilization of bovine oocytes with flow-cytometrically and unsorted sperm from different bulls.[J].Theriogenology 2001, 55:431.
  • 9Zhang M, Lu K H, Seidel G E. Development of bovine embryos after in vitro fertilization of oocytes with flow cytometrically sorted, stained and unsorted sperm from different bulls. [J]. Theriogenology, 2003, 60:1657-1663.
  • 10Li XH,Iwasiaki S and Nakahara T.Eeffects of trophctodem dissection on development of inner cell mass of fresh and frozen-thawed bovine blastocusts derived from in-vitro fertilization[J]. Journal of Reproduction and Development, 1993, 39:281-286.

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