摘要
以海带粉为原料,采用酶法提取海带蛋白,研究了酶添加量、料液比、pH、提取时间、温度等因素对海带蛋白提取率的影响。经单因素及正交试验得出海带蛋白的最佳提取条件为:液料比75,pH值11,提取时间5 h,酶添加量320 u/g。各因素对酶法提取海带蛋白的影响次序为:液料比>酶添加量>pH值>提取时间。
The enzymatic extraction of kelp protein was studied in this paper by controlling the ratio of water to stuff, pH, extraction time, extraction temperature, and the enzyme additive amount. Single factor test and orthogonal test were employed to optimize the extraction technique. The optimum conditions for enzymatic extraction were as follows ： pH 10, ratio of water to stuff 10 ： 1, pH 11, extraction time 5 hours, cellulase additive amount 320 u/g. The influencing factors had different effects on protein extraction in the following order： ratio of water to stuff 〉 enzyme additive amount 〉 pH 〉 time.
基金
山东省科技发展计划(2010GNC10917
2011GGH22110)
关键词
海带蛋白
酶法
提取工艺
kelp protein
enzymatic methods
extraction technique
