表达MDVgB基因重组痘苗病毒的构建及保护性研究

Construction of recombinant vaccina virus expressing glycoproteins B of Marke's disease virus and protection against it

以脂质体转染技术构建了表达鸡马立克氏病毒（ＭＤＶ）ｇＢ基因的重组痘苗病毒（ＲＶＶ－ｇＢ），表明该病毒在ＣＶ－１（猴肾细胞）细胞系内能稳定传代，经腹腔 １×１０（~6） ＰＦＵ·羽^（－１）将重组病毒、ＨＶＴ冻干苗及痘苗病毒分别按试验程序，免疫１日龄ＳＰＦ鸡，并于１５日龄以３×１０~２ＰＦＵ·羽^（－１）ＭＤＶ强毒株ＧＡ株攻毒，重组病毒和ＨＶＴ冻干苗匀获得较高的保护，结果证明了ｇＢ是ＭＤＶ的宿主保护性抗原．此研究为ＣＴＬ应答检测奠定了基础．

The recombinant vaccina virus (RVV) expressing Glycoproteins B of Marek's disease virus (MDVgB) were constructed by Lipofectin Regeant transfection This virus can be passaged stably in CV-1 cells. One-day-old SPF chickens were inoculated intraperitoneally with dose, 20~6 PFU of RVV, HVT freeze-drying-vaccine and vaccina virus respectively according to the procedure of experiment, and were challenged with dose 3 × 10~2 PFU of virulent MDV strain GA in their fifteen-days. High protection were acquired of both RVV and HVT freeze-drying-vaccine.These re- sults suggest that gB is a protective antigen of host animal. This study laid a foundation for cytotoxic T cell (CTL) dec- tection.