香鳞毛蕨不同提取液体外抗真菌作用研究

Study on Antifungal Susceptibility of Different Extract of Dryopteris fragrans

目的:对香鳞毛蕨不同提取液进行体外抗真菌试验,筛选其有效部位。方法:采用回流提取方法,用不同极性的溶剂对香鳞毛蕨进行提取,分为水、95%乙醇、石油醚和乙醚四种提取液,并测定其总间苯三酚的含量;通过纸片琼脂扩散法和试管内药基法研究不同提取液对临床常见的4种皮肤癣菌的最低抑菌浓度(MIC)和最低杀菌浓度(MFC),评价其体外抑菌效果。结果:香鳞毛蕨不同提取液的总间苯三酚含量依次为:95%乙醇提取液>水提取液>乙醚提取液>石油醚提取液;香鳞毛蕨不同提取液对所选菌种都具有一定的抗真菌作用,其体外抗真菌强度为:95%乙醇提取液>水提取液>乙醚提取液>石油醚提取液。结论:香鳞毛蕨95%乙醇提取液的总间苯三酚含量最高,体外抑菌活性最强,提示香鳞毛蕨抗真菌的有效部位为95%乙醇部位。

Objective：To examine the antifungal effect of different extract of Dryopteris fragrans（L.）Schott.in vitro,and screen the effective fraction from those extracts.Methods：Separated the Dryopteris fragrans extract and got four parts by refluxing extraction,and determined the contents of total phloroglucinol.Disc agar diffusion method and solid agar dilution method were used to determine inhibitory effect.Minimum inhibitory concentration（MIC） and minimal fungicidal concentration（MFC） of different parts of Dryopteris fragrans extract against four strains of common clinical dermatophytes were investigated.Results：The data showed that the contents sequence of total phloroglucinol was in the following order：95%-ethanol extractwater extractdiethyl ether extractpetroleum ether extract,and the antimicrobial activities against the four dermatophytes were as following order：95%-ethanol extract water extractdiethyl ether extractpetroleum ether extract.Conclusion：The contents of total phloroglucinol in 95%-ethanol extract of Dryopteris fragrans is the highest,and the antifungal activity against dermatophytes in vitro is the strongest.The effective fraction of Dryopteris fragrans is the 95%-ethanol extract.