水蛭素基因在酵母细胞中的分泌表达

Secretive Expression of Hirudin Gene in S.cerevisiae

报道了合成的水蛭素基因表达载体的构建 ,表达载体在酵母细胞中的表达 。

A DNA fragment coding for hirudin was inserted into the expression vector pYX212,with signal peptide α factor of S.cerevisiae.The hybrid protein was expressed and secreted into the medium,most of expressed antithrobin activity existed in the cell culture.