辛伐他汀对人牙髓干细胞增殖和分化的影响

Effects of simvastatin on the proliferation and osteoblastic differentiation of human dental pulp stem cells

目的:研究辛伐他汀对人牙髓干细胞(dental pulpstem cells,DPSCs)增殖和成骨分化的影响。方法:将第3代人DPSCs在矿化培养液中诱导培养,同时加入不同浓度的辛伐他汀(1×10-5mol/L、1×10-6mol/L、1×10-7mol/L、1×10-8mol/L),噻唑蓝(methyl thiazolyl tetrazolium,MTT)法检测细胞增殖情况,碱性磷酸酶试剂盒检测碱性磷酸酶(alkaline phosphatase,ALP)活性,茜素红染色鉴定成骨分化。结果:各浓度辛伐他汀均抑制人DPSCs增值,辛伐他汀浓度为1×10-5mol/L时,抑制作用最明显。适宜浓度辛伐他汀(1×10-6mol/L、1×10-7mol/L、1×10-8mol/L)促进人DPSCs向成骨细胞分化,其中,1×10-7mol/L的辛伐他汀促进ALP活性的作用最明显。结论:辛伐他汀抑制人DPSCs的增值,适宜浓度的辛伐他汀可有效促进人DPSCs的成骨分化。

differentiation of human dental pulp stem cells （DPSCs） were cultured in conditioned mineralization medium Methods The third passage human DPSCs with different concentrations of simvastatin Proliferation ability was detected by MTT method.Alkaline phosphatase activity was measured with ALP kit. Results Proliferation of human DPSCs was suppressed in all treatment groups with different concentrations of simvastatin（1×10^-5m01/L、l×10^-6mol/L、1×10^-7mol/L、1×10^-8mol/L） The maximum effect on the proliferation of DPSCs was observed at the concentration of 1×10^-5mol/L Osteoblastic differentiation of human DPSCs was increased in treatment group with different concentrations of simvastatin（1×10^-6mol/L、1x10^-7mol/L、1×10^-8mol/L） The maximum effect on the activity of ALP was observed at the concentration of 1x10^-7mol/L Conclusion Proliferation of human DPSCs was suppressed by simvastatin.Optimal concentration of simvastatin can improve the osteoblastic differentiation of human DPSCs.