银屑病患者Jak/STAT信号传导通路基因表达的研究

Gene Expression of Jak/STAT Pathway in Patients with Psoriasis

目的:探讨角质形成细胞Jak/STAT信号传导通路上的STAT1、STAT3、SOCS1、SOCS3 4个相关基因在银屑病发病中的作用。方法:收集10例银屑病患者皮损区和非皮损区组织各一块,用实时荧光定量PCR检测组织角质形成细胞中STAT1、STAT3、SOCS1、SOCS3 mRNA的表达,计算各基因mRNA的相对表达水平,探讨其与银屑病皮损发生的关系。结果:银屑病患者皮损区角质形成细胞STAT1、STAT3、SOCS1、SOCS3基因的mRNA表达水平(△Ct值分别为-1.9±1.6、0.6±1.6、0.1±1.0、-0.6±1.1)较非皮损区(△Ct值分别为1.0±1.6、3.7±1.5、4.2±1.2、3.9±1.3)明显增高(P值均<0.01);银屑病患者中角质形成细胞STAT1、STAT3 mRNA的表达水平分别与SOCS1和SOCS3 mRNA的表达水平呈正相关(r=0.84、0.83,P值均<0.01)。结论:角质形成细胞Jak/STAT信号传导通路相关基因可能与银屑病发病有关,需要进行结构与功能相关性的研究以进一步明确。

Objective ： To explore the role of Jak/STAT pathway in RNA expression level in pa- tients with psoriasis. Methods： The expression levels of STAT1, STAT3, SOCS1, SOCS3 genes were valuated in keratinocytes from lesional and non-lesional skin of 10 patients with psoriasis by using real-time quantitative PCR technique. Relationships of the expression levels of STAT1, STAT3, SOCSI, SOCS3 mRNA to the pathogenesis of psoriasis were analyzed. Results ： Differ- ence between expression levels of STAT1, STAT3, SOCS1, SOCS3 mRNA in keratinoeytes of le- sional skin and internal reference standard GAPHDH Ct （ACt） were - 1.9 ± 1.6,0. 6 ± 1.6, 0. 1 ± 1.0, -0. 6 ± 1.1 respectively, which were significantly higher than that of non-lesional skin （ ACt were 1.0 ±1.6, 3.7 ± 1.5, 4. 2 ± 1.2, 3.9 ± 1.3 ） respectively. The expression levels of STAT1, STAT3 mRNA of keratinocytes was found highly correlate to the that of SOCS1, as well as SOCS3 mRNA （ r = 0. 84,0. 83, P 〈 0. 01 ）. Conclusion： Jak/STAT pathway is involved in the pathogenesis of psoriasis. Further investigation is required for the understanding of the function of these abnormally exoressed genes.