肾综合征出血热患者外周血中VEGF通路相关分子的检测

Changes of the Inflammation-Related Plasma Cytokines in Hemorrhagic Fever with Renal Syndrome

目的检测肾综合征出血热(HFRS)患者不同病期外周血中VEGF通路相关分子含量的变化,并分析其与病程中血管渗漏的关系,为研究HFRS的发病机制提供依据。方法用酶联免疫吸附法(ELISA)检测141例不同病期HFRS患者外周血中血管内皮生长因子(VEGF)、可溶性血管内皮生长因子受体2(sVEGFR2)、可溶性血管内皮生长因子受体1(sVEGFR1)、可溶性血管内皮钙黏蛋白(sVE-cadherin)4种分子的含量,同时将30例健康志愿者作为正常对照。结果 HFRS患者血浆VEGF含量在发热期、少尿期、多尿期均高于对照者。HFRS患者sVEGFR2在休克期、少尿期、多尿期均低于对照者,sVE-cadherin在低血压休克期、少尿期高于正常对照者,sVEGFR1在血浆中的含量很低,仅能在休克期检测到极微量的存在。上述结果差异均有统计学意义。结论 HFRS患者血管通透性增高与VEGF表达升高可能有关,sVEGFR2的表达下降增强了VEGF的功能。sVE-cadherin在疾病进程中含量与疾病严重程度成正比。

Objective To explore the changes of VEGF-mediated micro-vascular permeability involved plasma cytokines in patients of hemorrhagic fever with renal synodrome（HFRS）. Methods Plasma samples were collected from 141 HFRS patients of different disease stages and 30 healthy volunteers.The concentration of vascular endothelial growth factor （VEGF） ,soluble vascular endothelial growth receptor 2 （sVEGFR2） ,soluble vascular endothelial growth receptor 1 （sVEGFRI）, soluble VE-cadherin（sVE-cadherin） were measured by sandwich enzyme-linked imunosorbent assay （ELISA）. Results The concentrations of VEGF were higher at febrile, oliguric and polyuric of HFRS compared to the healthy volunteers.The concentrations of sVEGFR2 decreased at shock,oliguric and polyuric of HFRS compared to the healthy volunteers.The concentrations of sVE-cadherin were higher at shock and oliguric of HFRS compared to the healthy volunteers. The sVEGFR1 was not detected in the healthy people, but it could be measured in the acute phase of HFRS patients＇ plasma. Conclusions It is expectable that VEGF may contribute to the clinical course of HFRS, and sVEGFR2 may increase the function of VEGF on vascular permeability.sVE-cadherin levels were significantly increased at the severe phase of the disease.