活性调节细胞骨架蛋白siRNA慢病毒表达载体对杏仁核点燃大鼠学习记忆的影响

Act of Arc siRNA via lentivirus on the memory consolidation of amygdala-kindled rats

目的研究慢病毒介导的活性调节细胞骨架蛋白（Arc）siRNA对杏仁核点燃大鼠学习记忆功能的影响。方法60只雄性SD大鼠随机分3组，每组20只，在大鼠点燃后，将ArcsiRNA慢病毒表达载体注入大鼠背侧海马的载体注射组，注入慢病毒空载体的空载体注射组，以及注射生理盐水的单纯点燃组。避暗实验检测大鼠记忆保持能力，Morris水迷宫检测大鼠空间学习和记忆能力，荧光定量PCR、免疫印迹技术检测大鼠海马ArcmRNA以及蛋白的表达。结果载体注射组、空载体注射组以及单纯点燃组，避暗实验：穿梭次数分别为11．5±1．1、3．7±1．5、3．8±1．3，进入暗箱潜伏期分别为15±8、94±11、99±11；Morris水迷宫显示载体注射组较其他两组，空间学习、记忆能力明显下降；荧光定量PCR：ArcmRNA相对表达量分别为5．80±0．06、13．55±0．42、15．82±0．51；免疫印迹：Arc蛋白相对含量分别为0．108±0．007、0．416±0．029、0．418±0．016，载体注射组与其他两组比较ArcmRNA及蛋白表达明显下降（P〈0．05）。结论Arc表达下降，严重影响杏仁核点燃大鼠记忆保持能力及空间学习、记忆功能，Arc在杏仁核点燃大鼠学习记忆功能中起重要作用。

Objective To explore the memory consolidation related role of gene Arc in amygdala- kindled rats. Methods A total of 60 male amygdala-kindled rats were divided equally into 3 groups ：0. 9 % sodium chloride injection, null vector lenvirus injection and silencing lentivirus injection. Quantitative polymerase chain reaction （qPCR） and Western blot were performed to confirm the silencing efficiency of Arc. And the passive avoidance test and Morris＇ water maze were used to test the memory consolidation ability of rats in three groups. Results The memory consolidation ability became significantly impaired in the Arc silencing group in both of passive avoidance test and water maze comparing to the other groups. And no significant difference existed in memory consolidation ability between 0. 9 % sodium chloride and null vector lenvirus injection groups. Conclusion The ability of memory consolidation is impaired in Arc silencing amygdala-kindled rats and Arc may play a significant role in the consolidation of memory in amygdala-kindled rats.