新型冷冻载体Cell Sleeper在微量人精子冷冻中的应用

Cell Sleeper:A New Type of Cryopreservation Carrier for Small Quantity of Human Spermatozoa

目的:评价一种新型的冷冻载体cell sleeper用于微量人类精子的冷冻效果。方法:将60例患者的精液标本液化,PureSperm梯度离心处理后进行两部分实验。第一部分运用cell sleeper在不同液量(0.5μl,1μl和2μl)含有冷冻保护剂的液滴进行精子冷冻,液氮中冷冻2周,比较冻融前后各组精子存活率和活力,以找到cellsleeper用于微量精子冷冻的最佳液量条件。在第一部分实验摸索的液量条件下,进行第二部分cell sleeper中进行单精子冷冻,并与常规冻存管进行比较。结果:第一部分实验中,三组精子解冻后的活力和存活率均较冷冻前下降,但三组间解冻后精子存活率和活力比较无差异。第二部分实验中,cell sleeper组精子解冻后的回收率和存活率明显高于常规冻存管组。结论:cell sleeper是一种理想的微量精子冷冻载体,解冻后可保证较高的精子回收率和存活率。

Objective： To evaluate cell sleeper as a new type of cryopreservation carrier for small quanti- ty of human spermatozoa. Methods： After liquefaction and PureSperm eentrifugation of spermato- zoa from 55 male volunteers, the study was designed into two parts. First part： compare the via- bility and motility of frozen-thawed spermatozoa cryopreserved by cell sleeper in different volume sizes. Second part： compare the recovery and viability rate of frozen-thawed spermatozoa using cell sleeper and conventional cryotube. Results： In the first part, the viability and motility of post-thawed spermatozoa were significantly lower than that of pre-freezing spermatozoa, but no significant difference was found among post-thawed spermatozoa cryopreserved in different vol- ume sizes. In second part, the recovery rate and viability of post-thawed spermatozoa in cell sleeper group were significantly higher than that of conventional cryotube. Conclusion： Cell sleeper is ideal for cryopreservation for small quantity of human spermatozoa with high recovery rate and viability.