羊膜对兔眼小梁切除术后滤过泡的影响

Effect of Amniotic Membrane on Filtering Bleb after Trabeculectomy in Rabbit Eyes

目的:探讨羊膜对小梁切除术后滤过泡的影响。方法:对12只新西兰白兔双眼行小梁切除术,术中1眼于巩膜和巩膜瓣间放置6mm×4mm的羊膜植片,另一眼作为对照。术后1周、2周、3周检查滤过泡形态和功能,光镜下观察滤过泡下巩膜表面瘢痕形成情况。结果:实验组术后1、2、3周滤过泡轻微隆起弥散,有较好的滤过功能,对照组术后2、3周滤过泡区瘢痕形成,滤过功能差。组织切片观察显示实验组滤过泡未形成瘢痕,成纤维细胞少,但有炎性细胞浸润;对照组滤过泡区被增生的纤维组织所代替,成纤维细胞丰富。结论:羊膜能改善滤过泡功能,减少瘢痕形成。眼科学报2000;16:73-76。

Objective: To investigate the effects of amniotic membrane on filtering blebs after trabeculectomy. Methods: Twelve rabbits underwent bilateral trabeculectomies. One eye was randomly sutured preserved human amniotic membrane between the sclera and the scleral flap and the other eye served as the control. The filtering blebs and filtering function were observed at the 1st, 2nd and 3rd week after the operation. The scar development situation between bleb and episclera was observed under the light microscope. Results: The blebs in amniotic membrane group were eminence, effuse and had a good filtering function at the 1 st, 2nd and 3rd week after the operation. The blebs in control group were cicatrized and had a bad filtering function. Histological observation showed no scar development, less fibroblast cell and more inflammatory cells infiltration at the site of trabeculectomy in amniotic membrane group. On the contrary, the control group showed more fibroplasia and fibroblast hyperplasia. Conclusion: Amniotic membrane can reduce the scar, improve filtering function. Eye Science 2000; 16: 73 - 76.